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er addgene plasmid 18000  (Addgene inc)


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    Structured Review

    Addgene inc er addgene plasmid 18000
    Er Addgene Plasmid 18000, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/er+addgene+plasmid+18000/GFP-Bcl2-Cb5+(Plasmid+%2318000)/pm23836782-28-67-68
    Average 93 stars, based on 9 article reviews
    er addgene plasmid 18000 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: BH4 domain of bcl-2 protein is required for its proangiogenic function under hypoxic condition.
    Article Snippet: Cell cultures, transfections and treatments Human umbilical endothelial cells (HUVEC; PromoCell GmbH, Heidelberg, Germany) and human melanoma cell lines (M14 and A375SM-SC1) were cultured as previously reported (14). .. M14 and A375SM-SC1 cells were stably transfected using Lipofectamine (Invitrogen, Carlsbad, CA) with expression vectors encoding the human bcl-2 protein wild-type or deleted of BH4 domain (aa 1–36), or mutated in BH1 (G145E) or BH2 (W188A) domains (13), with Flag-tagged vector encoding the only BH4 domain of bcl-2 (aa 1–30) or with green fluorescent protein (GFP)-tagged vector encoding bcl-2 protein wild-type (Addgene plasmid 17999) or targeted to ER (Addgene plasmid 18000). ..

    Transfection:

    Article Title: BH4 domain of bcl-2 protein is required for its proangiogenic function under hypoxic condition.
    Article Snippet: Cell cultures, transfections and treatments Human umbilical endothelial cells (HUVEC; PromoCell GmbH, Heidelberg, Germany) and human melanoma cell lines (M14 and A375SM-SC1) were cultured as previously reported (14). .. M14 and A375SM-SC1 cells were stably transfected using Lipofectamine (Invitrogen, Carlsbad, CA) with expression vectors encoding the human bcl-2 protein wild-type or deleted of BH4 domain (aa 1–36), or mutated in BH1 (G145E) or BH2 (W188A) domains (13), with Flag-tagged vector encoding the only BH4 domain of bcl-2 (aa 1–30) or with green fluorescent protein (GFP)-tagged vector encoding bcl-2 protein wild-type (Addgene plasmid 17999) or targeted to ER (Addgene plasmid 18000). ..

    Expressing:

    Article Title: BH4 domain of bcl-2 protein is required for its proangiogenic function under hypoxic condition.
    Article Snippet: Cell cultures, transfections and treatments Human umbilical endothelial cells (HUVEC; PromoCell GmbH, Heidelberg, Germany) and human melanoma cell lines (M14 and A375SM-SC1) were cultured as previously reported (14). .. M14 and A375SM-SC1 cells were stably transfected using Lipofectamine (Invitrogen, Carlsbad, CA) with expression vectors encoding the human bcl-2 protein wild-type or deleted of BH4 domain (aa 1–36), or mutated in BH1 (G145E) or BH2 (W188A) domains (13), with Flag-tagged vector encoding the only BH4 domain of bcl-2 (aa 1–30) or with green fluorescent protein (GFP)-tagged vector encoding bcl-2 protein wild-type (Addgene plasmid 17999) or targeted to ER (Addgene plasmid 18000). ..

    Plasmid Preparation:

    Article Title: BH4 domain of bcl-2 protein is required for its proangiogenic function under hypoxic condition.
    Article Snippet: Cell cultures, transfections and treatments Human umbilical endothelial cells (HUVEC; PromoCell GmbH, Heidelberg, Germany) and human melanoma cell lines (M14 and A375SM-SC1) were cultured as previously reported (14). .. M14 and A375SM-SC1 cells were stably transfected using Lipofectamine (Invitrogen, Carlsbad, CA) with expression vectors encoding the human bcl-2 protein wild-type or deleted of BH4 domain (aa 1–36), or mutated in BH1 (G145E) or BH2 (W188A) domains (13), with Flag-tagged vector encoding the only BH4 domain of bcl-2 (aa 1–30) or with green fluorescent protein (GFP)-tagged vector encoding bcl-2 protein wild-type (Addgene plasmid 17999) or targeted to ER (Addgene plasmid 18000). ..



    Similar Products

    93
    Addgene inc er addgene plasmid 18000
    Er Addgene Plasmid 18000, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/er+addgene+plasmid+18000/GFP-Bcl2-Cb5+(Plasmid+%2318000)/pm23836782-28-67-68
    Average 93 stars, based on 1 article reviews
    er addgene plasmid 18000 - by Bioz Stars, 2026-09
    93/100 stars
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